(25R)-16β,26-Dihydroxycholesterol is an intermediate in the synthesis of (25R)-26-Hydroxy-cholest-4-en-3-one (H917940), which is an oxygenated sterol which has many important biological activities. (25R)-26-Hydroxy-cholest-4-en-3-one has been used as a reactant for the synthesis of other sterols.